| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10802320 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2013 | 11 Pages |
Abstract
⺠In vivo evaluation of genetically encoded Ca2 + indicators (GECIs) is an important but time-taking procedure. ⺠Usually, a preliminary in vitro evaluation is performed, to determine their properties. ⺠These properties of GECIs, dynamic range, affinity, selectivity, kinetics, are described.
Keywords
Fractional saturationΔFGFPSNREGTAcDNAComplementary DNAEDTAethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acidFörster resonance energy transferbioluminescence resonance energy transferFRETBRETDissociation constantRatiometricFluorescence intensityDynamic rangeSignal-to-noise ratioFluorescent proteingreen fluorescent proteinFluorescent proteins
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Authors
Vadim Pérez Koldenkova, Takeharu Nagai,