Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10802357 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2013 | 9 Pages |
Abstract
Here we show the crucial role of MPP1 in lateral membrane ordering/organization in HEL cells (derived from erythroid precursors). Biochemical analyses showed that inhibition of MPP1 palmitoylation or silencing of the MPP1 gene led to a dramatic decrease in the DRM fraction. This was accompanied by a reduction of membrane order as shown by fluorescence-lifetime imaging microscopy (FLIM) analyses. Furthermore, MPP1 knockdown significantly affects the activation of MAP-kinase signaling via raft-dependent RTK (receptor tyrosine kinase) receptors, indicating the importance of MPP1 for lateral membrane organization. In conclusion, palmitoylation of MPP1 appears to be at least one of the mechanisms controlling lateral organization of the erythroid cell membrane. Thus, this study, together with our recent results on erythrocytes, reported elsewhere (Åach et al., J. Biol. Chem., 2012, 287, 18974-18984), points to a new role for MPP1 and presents a novel linkage between membrane raft organization and protein palmitoylation.
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Authors
Agnieszka Biernatowska, Joanna Podkalicka, MichaÅ Majkowski, Anita Hryniewicz-Jankowska, Katarzyna Augoff, Karol Kozak, Jan Korzeniewski, Aleksander F. Sikorski,