| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10802391 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2012 | 16 Pages |
Abstract
⺠A monitoring system with high sensitivity FRET-based biosensors has been developed. ⺠The established system enabled us to detect the most minute dynamics of caspase activation in single cells. ⺠The kinetics, dynamics, and selectivity in apoptotic signaling pathways with activation of caspase-8 were redefined. ⺠The actual number of pro-apoptotic molecules required for extrinsic apoptotic signal transduction was evaluated. ⺠A mathematical model predicted the minimal concentration of caspase-8 required to act as an initiator.
Keywords
FRBeGFPz-IETD-FMKXIAPDEDCASPmRFP1PARPCHXFCSFADDDISCSmall interfering RNAsiRNACaspase cascadeFluorescence resonance energy transferFRETApoptosisdeath effector domainfetal calf serumcycloheximideFas LigandFasLTRAILtumor necrosis factor-related apoptosis-inducing ligandMathematical modelFAS-associated death domain proteinenhanced green fluorescent proteinPoly(ADP-ribose) polymeraseBIDdeath-inducing signaling complexcaspaseCarp
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Katsuya Kominami, Jun Nakabayashi, Takeharu Nagai, Yuki Tsujimura, Kumiko Chiba, Haruna Kimura, Atsushi Miyawaki, Tatsuya Sawasaki, Hideo Yokota, Noboru Manabe, Kazuhiro Sakamaki,