The enhanced host-cell permissiveness of human cytomegalovirus is mediated by the Ras signaling pathway

Article ID	Journal	Published Year	Pages	File Type
10802752	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research	2011	11 Pages	PDF

Abstract

âº H-ras-transformed cells show increased permissiveness to HCMV compared to their parental non-transformed cells. âº Inhibition of the Ras pathway, effectively suppresses viral infection in the H-ras-transformed cells. âº HCMV promotes cellular foci formation and increased proliferation rate of the H-ras-transformed cells. âº HCMV-infected ras-transformed cells are more susceptible to apoptosis facilitating the efficient viral spread.

Keywords

HHV ERK RAS HSV PI3-K HFF FTi VZV PKR EGFR HCMV RNA-activated protein kinase TNF α MAPKK MAPKKK RalGDS nuclear factor kappa light chain enhancer of activated B cells PAA DMEM SDS FBS EGF NF-κB IPTG hpi MAPK MAPK kinase MAPK kinase kinase MOI Dulbecco's modified Eagle's medium Mitogen activated protein kinases EBV Transformation late Early hours post-infection Cancer fetal bovine serum Human cytomegalovirus epidermal growth factor tumor necrosis factor Phosphatidylinositol 3-kinase immediate early human foreskin fibroblasts Farnesyl transferase inhibitor Signal transduction plaque-forming units Epstein-Barr virus human herpes virus herpes simplex virus Varicella zoster virus pfu multiplicity of infection extracellular regulated kinase Epidermal growth factor receptor