Extracellular ATP induces spikes in cytosolic free Ca2+ but not in NADPH oxidase activity in neutrophils

Article ID	Journal	Published Year	Pages	File Type
10802792	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research	2011	7 Pages	PDF

Abstract

âº In this study we have measured Ca2+ and NADPH oxidase activity in neutrophils. âº ATP induces spikes in cytosolic Ca2+ concentration. âº NADPH oxidase does not show rapid spikes. âº Ca2+ is an on-switch for NADPH oxidase.

Keywords

N-formyl-l-methionyl-l-leucyl-l-phenylalanine fMLP Phorbol-12-myristate-13-acetate HRP PKC Ca2+PMA [Ca2+]cyt ATP NADPH oxidase Peripheral blood diacyl glycerol DAG Spikes Cytosolic Ca2+ concentration Neutrophils Hydroethidine Horseradish peroxidase Protein kinase C Calcium