Phosphorylated c-Jun and Fra-1 induce matrix metalloproteinase-1 and thereby regulate invasion activity of 143B osteosarcoma cells

Article ID	Journal	Published Year	Pages	File Type
10802807	Biochimica et Biophysica Acta (BBA) - Molecular Cell Research	2011	11 Pages	PDF

Abstract

âº Osteosarcoma patients develop pulmonary metastases, but its mechanisms remain unclear. âº MMP-1 and CXCR4 were notably expressed in a highly metastatic osteosarcoma cell line. âº MAPK pathways activated AP-1 (c-Jun/Fra-1), and thereby regulated MMP-1 expression. âº The MAPK/AP-1/MMP-1 axis plays a central role in osteosarcoma invasion.

Keywords

SDF-1α RT-PCR ERK ETs ECM FCS AP-1 CXCR4 c-Jun NH2-terminal kinase MNNG MMP-1 FRA-1 Pea3 WST-8 IL-2Rα E-twenty six EMSA GAPDH NF-κB MMP PBS Jnk MAPK N-methyl-N′-nitro-N-nitrosoguanidine Small interfering RNA siRNA Electrophoretic mobility shift assay Osteosarcoma standard deviation interleukin Enzyme-linked immunosorbent assay ELISA Invasion c-Jun fetal calf serum Stromal cell-derived factor-1α nuclear factor-κB Extracellular matrix matrix metalloproteinase Pulmonary metastasis Phosphate-buffered saline reverse transcription-PCR wild type retinoblastoma protein activator protein-1 mitogen-activated protein kinase extracellular signal-regulated kinase glyceraldehyde 3-phosphate dehydrogenase