| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10802882 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2011 | 10 Pages |
Abstract
⺠The ubiquitous Ca2+ ATPases SERCA2b and SPCA1a so far evaded purification. ⺠A new purification strategy was developed biasing towards properly folded protein. ⺠This method uses thapsigargin and reactive dye as ligands. ⺠Elution of WT hSERCA2b was hampered by its nM affinity for thapsigargin. ⺠Thapsigargin mutant hSERCA2b-I765A could be easily purified.
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Authors
Ilse Vandecaetsbeek, Søren Brøgger Christensen, Huizhen Liu, Paul P. Van Veldhoven, Etienne Waelkens, Jan Eggermont, Luc Raeymaekers, Jesper V. Møller, Poul Nissen, Frank Wuytack, Peter Vangheluwe,