| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10802897 | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research | 2011 | 10 Pages |
Abstract
⺠FTIR microspectroscopy has been applied to the study of single Surrounded Nucleolus (SN) and Not Surrounded Nucleolus (NSN) murine oocytes at three stages of their maturation. ⺠The two types of oocytes displayed differences in the lipid, protein, and in the nucleic absorption regions. ⺠The most significant differences concerned the IR bands reflecting transcriptional activity and polyadenylation. ⺠The multivariate statistical analysis allowed to identify the maturation stage where the separation between the two types of oocytes takes place.
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Authors
Diletta Ami, Paolo Mereghetti, Antonino Natalello, Silvia Maria Doglia, Mario Zanoni, Carlo Alberto Redi, Manuela Monti,