Article ID Journal Published Year Pages File Type
10802917 Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2010 7 Pages PDF
Abstract
Importin α is a nuclear transport receptor well established for its ability to mediate importin β-mediated nuclear import of proteins that possess classical nuclear localization signal (cNLS). Previously, we reported that importin α rapidly accumulates to the nucleus in response to H2O2-induced oxidative stress, which implies a role for this protein in stress response. In this study, we show that importin α1 (also known as KPNA2 or Rch1), a major subtype of the importin α family, localizes to RNA stress granules (SGs), large cytoplasmic bodies that are thought to function as RNA triage sites during stress response. The recruitment of importin α1 to SGs was compatible with its nuclear accumulation during heat shock. Depletion of endogenous importin α1 using siRNA showed that importin α1 regulates the dynamics of SG assembly, and that it promotes cell survival in arsenite-treated cells. These data revealed, for the first time, the involvement of importin α in the assembly of RNA granules and its pro-survival role during stress response.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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