Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10803760 | Biochimie | 2013 | 5 Pages |
Abstract
⺠We investigated ABCA1 molecular function by tagging and isolating the protein. ⺠The 1D4 tag did not modify the protein expression/localization within the cells. ⺠The 1D4 tag did not impair ABCA1-dependent cholesterol efflux to apo A-I. ⺠Solid-phase binding assays showed no direct binding of cholesterol to ABCA1. ⺠Solid-phase binding assays showed no direct binding of apo A-I to ABCA1 either.
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Authors
Emmanuelle Reboul, Frank M. Dyka, Faraz Quazi, Robert S. Molday,