Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10803784 | Biochimie | 2013 | 6 Pages |
Abstract
In this study, the antifungal activity and mode of action(s) of hibicuslide C derived from Abutilon theophrasti were investigated. Antifungal susceptibility testing showed that hibicuslide C possessed potent activities toward various fungal strains and less hemolytic activity than amphotericin B. To understand the antifungal mechanism(s) of hibicuslide C in Candida albicans, flow cytometric analysis with propidium iodide was done. The results showed that hibicuslide C perturbed the plasma membrane of the C. albicans. The analysis of the transmembrane electrical potential with 3,3â²-dipropylthiacarbocyanine iodide [DiSC3(5)] indicated that hibicuslide C induced membrane depolarization. Furthermore, model membrane studies were performed with calcein encapsulating large unilamellar vesicles (LUVs) and FITC-dextran (FD) loaded LUVs. These results demonstrated that the antifungal effects of hibicuslide C on the fungal plasma membrane were through the formation of pores with radii between 2.3 nm and 3.3 nm. Finally, in three dimensional flow cytometric contour plots, a reduced cell sizes by the pore-forming action of hibicuslide C were observed. Therefore, the present study suggests that hibicuslide C exerts its antifungal effect by membrane-active mechanism.
Keywords
PBS3,3′-dipropylthiacarbocyanineFITC–dextranKCTCCH2Cl2EtOAcCLSIATCCMICFITC3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromideDiSC3(5)MTTEthyl acetateAntifungal effectlarge unilamellar vesicleMinimum inhibitory concentrationDichloromethanePhosphate buffered salinephosphatidylinositolphosphatidylcholinephosphatidylethanolaminefluorescein isothiocyanatePhytochemicalLUVMethanolAmerican Type Culture CollectionKorean Collection for Type CulturesClinical and Laboratory Standards InstituteMeOHCandida albicans
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Authors
Ji Hong Hwang, Qinglong Jin, Eun-Rhan Woo, Dong Gun Lee,