| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10803801 | Biochimie | 2012 | 9 Pages |
Abstract
⺠Dictyoglomus turgiduml-fucose isomerase catalyzes l-fucose isomerization. ⺠The enzyme has the highest activity among l-fucose/d-arabinose isomerases. ⺠Glu349, Asp373, and His539 were identified as metal-binding residues. ⺠Val131, Met197, Ile199, Gln314, Ser405, Tyr451, and Asn538 were substrate-binding residues. ⺠Arg30, Trp102, Asn404, Phe452, and Trp510 were activity-related residues.
Keywords
AASSodium dodecyl IPTGEPPSPAGEpiperazine-N,N′-bis(2-ethanesulfonic acid)EDTAEthylenediaminetetraacetic acidpolyacrylamide gel electrophoresisisopropyl-β-d-thiogalactopyranosideCharacterizationMolecular dynamicsAtomic absorption spectroscopyinductively coupled plasma mass spectrometryICP-MSLuria-BertaniPipes
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Authors
Seung-Hye Hong, Yu-Ri Lim, Yeong-Su Kim, Deok-Kun Oh,