The thermal stability of the external invertase isoforms from Saccharomyces cerevisiae correlates with the surface charge density

► We examined differences in the thermal stability of the external invertase isoforms. ► Phosphorylated polymannan component is responsible for differences between isoforms. ► Distribution of negative surface charge influences differences in thermal stability. ► Charged groups bound to the glyco-component can influence stability of glycoproteins.