| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10803929 | Biochimie | 2013 | 9 Pages |
Abstract
⺠Reaction of HeLa cell extract with circular duplex substrates containing a long gap induces efficient DNA synthesis in vitro. ⺠We constructed a mammalian cell-free system in which radiolabelling is not required, and DNA synthesis is quantifiable. ⺠In this system, physiological DNA replication, particularly the elongation process, may be reproduced. ⺠Quantitative assay of mammalian DNA replication may provide various advantages.
Keywords
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Seiki Nakao, Sufang Zhang, Markku Vaara, Juhani E. Syväoja, Marietta Y. Lee, Toshiki Tsurimoto, Peter Karran, Shinya Oda,