| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10803965 | Biochimie | 2012 | 4 Pages |
Abstract
⺠Three-site-directed active-site mutants of the hsPLA2GIID have been prepared using site-directed mutagenesis. ⺠All mutants have been successfully expressed and refolded to the native conformation. ⺠Bactericidal activity of the protein has been directly demonstrated. ⺠A calcium-independent (and hydrolysis independent) membrane-damaging effect of the enzyme is described. ⺠The hydrolysis independent membrane-damaging effect is relevant for the bactericidal mechanism of the protein.
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Authors
Raquel Fonseca-Maldonado, Tatiana Lopes Ferreira, Richard J. Ward,