| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10803988 | Biochimie | 2013 | 9 Pages |
Abstract
⺠Dietary oxysterol cytotoxicity was investigated in colon cancer CaCo-2 cells. ⺠They were applied to the cells individually or in mixture as present in the diet. ⺠Oxysterols used individually are more toxic than in mixture in inducing apoptosis. ⺠α-epoxycholesterol and 7-hydroxycholesterols are the most effective. ⺠Specific oxysterols may induce selective cytotoxicity against colon cancer cells.
Keywords
2′,7′-dichlorofluorescin-diacetateECLAmCDPIDCFH-DAIBDHRPNADPHDcfHEPESFITC7α-OHNoxA15β,6β-epoxycholesterolNoxO1Nox Activator 1PBS7β-OHα-EPOXCHOLDMEMFBS2′,7′-dichlorofluorescein4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid5α,6α-epoxycholesterol7-amino-4-methylcoumarin7-Ketocholesterol7β-hydroxycholesterolAc-DEVD-AMCDulbecco's modified Eagle's mediumMTTNOxROSEDTAEthylenediaminetetraacetic acidOxysterolsOXYNADPH oxidaseenhanced chemiluminescenceInflammatory bowel diseaseApoptosisdiphenylene iodoniumColon cancerfoetal bovine serumCaco-2 cellsPhosphate buffered salinefluorescein isothiocyanateLDH, lactate dehydrogenasenicotinamide adenine dinucleotide phosphate reduced7α-hydroxycholesterolDHEHorseradish peroxidasecholesterolReactive oxygen species
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Authors
Fiorella Biasi, Elena Chiarpotto, Barbara Sottero, Marco Maina, Cinzia Mascia, Tina Guina, Paola Gamba, Simona Gargiulo, Gabriella Testa, Gabriella Leonarduzzi, Giuseppe Poli,