Glucose oxidase induces insulin resistance via influencing multiple targets inÂ vitro and inÂ vivo: The central role of oxidative stress

Article ID	Journal	Published Year	Pages	File Type
10804051	Biochimie	2012	13 Pages	PDF

Abstract

âº GOD increased fasting blood glucose and damaged glucose and insulin tolerance in rats. âº GOD disrupted insulin signaling both in rats and in hepatocytes. âº GOD damaged Nrf2-regulated antioxidant enzymes and increased ROS production. âº ROS played a central role in GOD-induced insulin resistance inÂ vitro and vivo.

Keywords

Heme oxygenase-1 NAC GPX HO-1 ERK PPARγ N-acetyl-l-cysteine PGC-1α IRS-1 RT-PCR GRP78 T2DM GCLC GLUT2 IPITT c-jun amino-terminal kinase catalytic subunit of γ-glutamylcysteine ligase GSK3α/β Nrf2 CAT Jnk FBG AUC IPGTT MAPK ROS intraperitoneal insulin tolerance test intraperitoneal glucose tolerance test Glucose oxidase Akt insulin receptor substrate-1 Oxidative stress GOD Type 2 diabetes mellitus SOD Superoxide dismutase endoplasmic reticulum fasting blood glucose Insulin resistance area under the curve protein kinase B mitogen-activated protein kinase Catalase extracellular signal-regulated kinases glutathione reductase glutathione peroxidase glucose transporter 2 Reactive oxygen species