| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10804105 | Biochimie | 2012 | 8 Pages |
Abstract
⺠Isothermal titration calorimetry analysis indicated 1 calcium-binding site in actinomycete mannanases. ⺠The calcium-binding site is near the catalytic cleft. ⺠One calcium binding site of StMan affects thermal stability and hydrolysis activity. ⺠One calcium binding site of TfMan affects only thermal stability. ⺠Successive calcium binding residues are conserved in bacterial GH5 mannanase.
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Authors
Yuya Kumagai, Kayoko Kawakami, Takafumi Mukaihara, Masayo Kimura, Tadashi Hatanaka,