| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10804132 | Biochimie | 2012 | 11 Pages |
Abstract
⺠Detailed insights into the degradation of elastin by serine elastases. ⺠HLE, PR3 and CG tolerate hydrophobic and/or aliphatic amino acids. ⺠CG also prefers bulky amino acids including Leu, Lys, Phe and Tyr. ⺠The three elastases release matrikines, with CG producing the highest number. ⺠Peptides containing the motifs GVYPG, GFGPG and GVLPG stimulate pro-MMP-1 secretion.
Keywords
ECMQqTOFTRISPBSPR3EBPEDPPAGENSPTFAACN2-amino-2-(hydroxymethyl)-1,3-propanediolAcetonitrileTrifluoroacetic acidFormic acidpolyacrylamide gel electrophoresisHuman Leukocyte ElastaseEnzyme-linked immunosorbent assayELISAMass spectrometryPhosphate buffered salineExtracellular matrixMALDIHLEproteinase 3cathepsin Ghigh performance liquid chromatographyHPLC
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Authors
Andrea Heinz, Michael C. Jung, Günther Jahreis, Anthony Rusciani, Laurent Duca, Laurent Debelle, Anthony S. Weiss, Reinhard H.H. Neubert, Christian E.H. Schmelzer,