| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10804203 | Biochimie | 2011 | 9 Pages |
Abstract
⺠Methanogenic-type SerRS has a highly flexible motif (SOL) within the active site. ⺠Notable structural rearrangements of the SOL peptide parallel Ser-tRNASer formation. ⺠SOL may play the role in mediation of open vs closed active site conformation. ⺠Coupling of the SOL conformational change with active site assembly occurs via Trp396. ⺠His250, a non-SOL residue, is essential for transfer of serine to tRNA.
Keywords
aminoacyl-adenylateGlnRSHisRSTrpRSThrRSp-hydroxymercuribenzoate4-(2-Pyridylazo)-resorcinolSERAMstRNASerSeryl-tRNA synthetaseSERRSPEIaa-AMPHistidyl-tRNA synthetasePMBtRNAITCGlutaminyl-tRNA synthetaseAminoacyl-tRNA synthetasetransfer RNAThreonyl-tRNA synthetaseTryptophanyl-tRNA synthetaseParActive site mutantsaaRSSolInorganic pyrophosphatasepolyethyleneimineIsothermal titration calorimetry
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Authors
Morana Dulic, Josip Pozar, Silvija Bilokapic, Ivana Weygand-Durasevic, Ita Gruic-Sovulj,