Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10804218 | Biochimie | 2011 | 12 Pages |
Abstract
⺠Core-domain residue affecting RNase A aggregation. ⺠S80R mutation increases N-terminal swapping RNase A multimerization. ⺠S80R mutant forms multimers more stable than wt ones.
Keywords
SECo.n.BS-RNaseESI-TOF MSRibonuclease AddH2OWtsPMSFPAGERP-HPLCNAPIPTGFPLCAMPTFAdouble-distilled waterAmpicillinTrifluoroacetic acidpolyacrylamide gel electrophoresisSize-exclusion chromatographyprotein aggregationcircular dichroismfast performance liquid chromatographySodium phosphatephenylmethylsulfonyl fluoride3D domain swappingreverse-phase high pressure liquid chromatographyovernight
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Authors
Francesca Vottariello, Enrico Giacomelli, Roberta Frasson, Nicola Pozzi, Vincenzo De Filippis, Giovanni Gotte,