| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10804303 | Biochimie | 2011 | 7 Pages |
Abstract
⺠Two permuted linear genes were constructed by circular permutation of the as-48A structural gene. ⺠None of the leaderless linear proteins could be detected in E. coli BL21 (DE3) extracts. ⺠Protein fusions to an appropriate partner favour the intracellular accumulations as inclusion bodies. ⺠Cleavage of the fusion proteins yields hybrids proteins with antimicrobial activity. ⺠The AS48/49-fusion protein was detected in a much lower amount compared with AS23/24.
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Authors
Manuel Montalbán-López, Manuel MartÃnez-Bueno, Eva Valdivia, Mercedes Maqueda,