Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10804370 | Biochimie | 2005 | 8 Pages |
Abstract
YCP, a mitogenic polysaccharide with its molecular weight (MW) of 2.4 Ã 103 kDa, was isolated from the mycelium of the marine filamentous fungus Phoma herbarum YS4108 by a combination of ion-exchange chromatography on DEAE-32 and gel permeation over Sephacryl S-400. The detailed compositional, spectroscopic and methylation analyses of the polysaccharide demonstrated that its backbone possessed most likely a linear α-(1 â 4) bonded glucopyranoside main chain co-bearing through side α-(1 â 6)-linkage. The α-(1 â 4) bondage of the glucopyranoside building blocks in YCP was confirmed by the observation that it could be hydrolyzed by the α-amylase produced by Bacillus licheniformis. A reliable concentration monitoring experimentation highlighted that the reducing sugars released continuously from YCP during its incubation with the enzyme, and the MW of the main resulting fragment weighed 0.8 à 104 Da with approximately 10% of YCP converted to maltose, maltotriose and glucose after a 120-min enzymatic degradation. Finally, YCP was found to be able to increase phagocytic activity of mice in vitro and in vivo, indicating that it may be looked up as a potent immunomodulator that could activate macrophages.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
X.B. Yang, X.D. Gao, F. Han, B.S. Xu, Y.C. Song, R.X. Tan,