Selective interactions of spinophilin with the C-terminal domains of the Î´- and Î¼-opioid receptors and G proteins differentially modulate opioid receptor signaling

Article ID	Journal	Published Year	Pages	File Type
10815408	Cellular Signalling	2012	14 Pages	PDF

Abstract

âº Direct physical association of Î´-opioid receptor with spinophilin in HEK293 cells âº Î´-Î¼-opioid receptors differentially couple with spinophilin. âº The COOH tail of opioid receptors docking site of spinophilin âº Spinophilin GÎ±, GÎ²Î³, RGS4 and opioid receptors form a protein complex. âº Î´-Î¼-opioid receptor signaling is differentially altered by spinophilin.

Keywords

μ-CT GEFs DPDPE RGS [d-Pen2,d-Pen5]-enkephalin GPCR NRS PMSF hemaglutinin ERK GST IBMX 6xHIS IPTG PBS NMS Regulators of G protein signaling DSLET 1-methyl-3-isobutylxanthine DAMGO G protein-coupled receptors MAPK δ-Opioid receptor μ-Opioid receptor adenylyl cyclase Spinophilin Amino acids SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis isopropyl β-D-1-thiogalactopyranoside Protein–protein interactions normal rabbit serum normal mouse serum Signaling complex Guanine nucleotide exchange factors Polyvinylidene difluoride membrane PVDF membrane phenylmethylsulfonyl fluoride Phosphate-buffered saline hexahistidine G proteins mitogen activated protein kinase Extracellular signal-regulated protein kinase extracellular signal-regulated kinases glutathione-S-transferase