Raft coalescence and FcγRIIA activation upon sphingomyelin clustering induced by lysenin

► Binding of lysenin to the plasma membrane induces clustering of raft sphingomyelin. ► Upon sphingomyelin aggregation, non-activated FcγRIIA associates with rafts. ► Sphingomyelin clustering triggers phosphorylation of FcγRIIA and signaling proteins. ► Complexes of activated FcγRIIA coalesce after sphingomyelin clustering. ► Raft merging evoked by sphingomyelin aggregation induces signaling of FcγRIIA.