Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10815654 | Cellular Signalling | 2010 | 13 Pages |
Abstract
The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. β-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active β-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low β-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high β-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/β-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment.
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Authors
Ersen Kavak, Ayaz Najafov, Nuri Ozturk, Tuncay Seker, Kader Cavusoglu, Tolga Aslan, Adil Doganay Duru, Tahsin Saygili, Gerta Hoxhaj, Mahmut Can Hiz, Durisehvar Ozer Unal, Necla Birgül-İyison, Mehmet Ozturk, Ahmet Koman,