Phosphorylation on the Ser 824 residue of TRPV4 prefers to bind with F-actin than with microtubules to expand the cell surface area

► We presumed the phosphorylation on Ser 824 in TRPV4 as the regulatory point. ► The properties of a phospho-mimicking TRPV4 mutant were examined. ► The phosphorylation on the Ser 824 seems to be a major modification.