Characterization of the interaction between the dopamine D4 receptor, KLHL12 and Î²-arrestins

Article ID	Journal	Published Year	Pages	File Type
10815878	Cellular Signalling	2016	41 Pages	PDF

Abstract

Finally, our results indicate that p44/42 MAPK phosphorylation, the signaling pathway which is often regulated by Î²-arrestins is not influenced by KLHL12, but seems to be exclusively mediated by GÎ±i protein upon dopamine D4 receptor stimulation.

Keywords

PBS IC3 β-arrestin DUB V2R β2AR USP BTB GRK CXCR4 PTX PKC RIPA GPCR VNTR DMEM N-ethylmaleimide JNK3 pKa PEI G protein-coupled receptor kinase MAPK Dulbecco's modified Eagle's medium deubiquitinating enzyme attention deficit hyperactivity disorder Immunoblotting radioimmunoprecipitation buffer ADHD Variable number of tandem repeats third intracellular loop pertussis toxin Co-IP Phosphate-buffered saline NEM C-X-C chemokine receptor type 4 Co-Immunoprecipitation ubiquitin-specific protease protein kinase A Protein kinase C mitogen-activated protein kinase Polyethylenimine Dopamine D4 receptor β2-adrenergic receptor Vasopressin V2 receptor G protein-coupled receptor ubiquitination