Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10816075 | Cellular Signalling | 2014 | 7 Pages |
Abstract
HIF-1α is implicated in hepatocellular carcinoma (HCC) pathologies. Here, we screened a human liver cDNA library for HIF-1α-interacting partners using a yeast two-hybrid system. We identified 53 genes, including formiminotransferase cyclodeaminase (FTCD), which was confirmed by co-immunoprecipitation. Moreover, our data indicated that HIF-1α mediated the effects of hypoxia on FTCD induction via binding to the hypoxia-responsive elements of the FTCD promoter. Knockdown of FTCD reduced the effects of HIF-1α in hypoxia and enhanced chemosensitivity in HepG2 cells. Our findings suggested crosstalk between FTCD and HIF signaling and promoted HCC progression, thus implicating FTCD as a therapeutic target for HCC.
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Authors
Zhenhai Yu, Yingying Ge, Lei Xie, Teng Zhang, Liangqian Huang, Xiaoping Zhao, Jianjun Liu, Gang Huang,