| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10816331 | Cellular Signalling | 2012 | 10 Pages |
Abstract
⺠Mutating five of 21 amino acids in the PAR1-i2 loop reduce PAR1-Gq/11 signalling. ⺠None of the mutants affect PAR1 binding or functional coupling to Gi/o or G12/13. ⺠Mutation R205A alone prevents PAR1 complex formation with G11 but not G12 or Go. ⺠Mutation R205A blocks PAR1 InsP/Ca++ signaling in astrocytes. ⺠PAR1 calcium response in astrocytes involves a Gq/11-PLCe signalling pathway.
Keywords
PLCPLCεtris-buffered saline-TweenInsPG12/13p63RhoGEFGRK2PTXTBS-TPBSGPCRECLERKDMEMBSAG11inositol phosphateMAPKDulbecco's modified Eagle's mediumbovine serum albuminenhanced chemiluminescenceThrombinParG proteinBBBBlood–brain barrierpertussis toxinphospholipase CPhosphate-buffered salinemitogen activated protein kinaseextracellular signal-regulated kinaseprotease-activated receptorG protein-coupled receptor
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Authors
Kelly L. McCoy, Stefka Gyoneva, Christopher P. Vellano, Alan V. Smrcka, Stephen F. Traynelis, John R. Hepler,