Increased interaction of connexin43 with zonula occludens-1 during inhibition of gap junctions by G protein-coupled receptor agonists

Article ID	Journal	Published Year	Pages	File Type
10816461	Cellular Signalling	2012	13 Pages	PDF

Abstract

âº We study the mechanisms that govern gap junction inhibition in astrocytes. âº Endothelin and sphingosine-1-phosphate trigger fast and drastic inhibition of gap junctions. âº Activation of G(i/o) is required for agonist-induced inhibition of gap junctions. âº Inhibition of gap junctions involves increase of molecular Cx43-ZO-1 interactions. âº Dephosphorylation of junctional Cx43 accompanies inhibition of gap junctions.

Keywords

ERK PTX PKC TBST S1P GPCR LPA GJIC TRITC HRP Connexin43 deoxyglucose CX43 IgG BSA MAPK MTT bovine serum albumin gap junctional intercellular communication horse-radish peroxidase Sphingosine-1-phosphate lysophosphatidic acid Sodium dodecylsulfate-polyacrylamide gel electrophoresis SDS-PAGE endothelin immunoglobulin G Thiazolyl blue tetrazolium bromide tetramethylrhodamine isothiocyanate Immunoprecipitation pertussis toxin gap junction Phosphatase GTP-binding protein Protein kinase C mitogen-activated protein kinase Carboxyl connexin extracellular signal-regulated kinase Rho-associated kinase Rock