The third intracellular loop of D1 and D5 dopaminergic receptors dictates their subtype-specific PKC-induced sensitization and desensitization in a receptor conformation-dependent manner

Article ID	Journal	Published Year	Pages	File Type
10816464	Cellular Signalling	2012	13 Pages	PDF

Abstract

âº D1R sensitization and D5R desensitization by PMA is not linked to AC modulation. âº IL3 but not CT imparts PMA-induced D1R sensitization and D5R desensitization. âº Intramolecular IL3 interactions shape PMA-induced regulation of D1R and D5R. âº PMA-induced regulation of D1R and D5R is dependent on receptor conformation.

Keywords

Phorbol-12-myristate-13-acetate ionomycin Emax FBS Bmax forskolin PKC PBS β2-AR IL3 Fsk EC50 D1-like receptors GPCR HEK293 BSA DMSO PMA adenylyl cyclase bovine serum albumin minimum essential medium Sensitization third intracellular loop Dopamine Dimethyl sulfoxide fetal bovine serum Cytoplasmic tail maximal binding capacity transmembrane MEM Phosphate-buffered saline Protein kinase C Desensitization human embryonic kidney 293 β2-adrenergic receptor G protein-coupled receptor Iono