Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10816627 | Cellular Signalling | 2005 | 17 Pages |
Abstract
2-(p-amylcinnamoyl)amino-4-chlorobenzoic acid (PACA), pharmacological inhibitor of phospholipase A2 (PLA2), inhibits epinephrine-stimulated thromboxane production in human platelets. In this study, we investigated the effect of PACA on meiotic maturation individually in stages V and VI oocytes. PACA prevented the maturation in stage V but merely delayed the process in stage VI oocytes. This was associated with the strong inhibition of Mos synthesis at both stages. Besides, PACA-induced inhibition of MAPK activation was evident in stage V but not in stage VI oocytes. PACA also inhibited the activation of Cdc2 kinase (Cdc2) in stage V but merely delayed the process in stage VI oocytes. Furthermore, 5 μM and higher concentrations of PACA completely inhibited the activation of MAPK and Cdc2 only in stage V, not in stage VI, oocytes. Moreover, we propose PACA as a new tool for the study of Xenopus oocyte maturation, which can also play a unique role for the studies of the stage-specific activation of MAPK and Cdc2.
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Authors
Azharul Islam, Yoko Sakamoto, Kazuhisa Kosaka, Satoshi Yoshitome, Isamu Sugimoto, Kazuo Yamada, Ellen Shibuya, George F. Vande Woude, Eikichi Hashimoto,