Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10820506 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2005 | 8 Pages |
Abstract
Selenium-containing glutathione transferase (seleno-GST) was generated by biologically incorporating selenocysteine into the active site of glutathione transferase (GST) from a blowfly Lucilia cuprina (Diptera: Calliphoridae). Seleno-GST mimicked the antioxidant enzyme glutathione peroxidase (GPx) and catalyzed the reduction of structurally different hydroperoxides by glutathione. Kinetic investigations reveal a ping-pong kinetic mechanism in analogy with that of the natural GPx cycle as opposed to the sequential one of the wild type GST. This difference of the mechanisms might result from the intrinsic chemical properties of the incorporated residue selenocysteine, and the selenium-dependent mechanism is suggested to contribute to enhancement of the enzymatic efficiency.
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Authors
Huijun Yu, Junqiu Liu, Xiaoman Liu, Tianzhu Zang, Guimin Luo, Jiacong Shen,