| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10820806 | Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology | 2005 | 7 Pages |
Abstract
Fructose 1,6-bisphosphatase (FBPase; EC 3.1.3.11) localization in cardiomyocyte nuclei has recently been investigated in mammals [FEBS Lett. 539 (2003) 51]. In this study, nuclear localization of FBPase in the cardiac muscle of the chicken was studied by immunohistochemistry and other methods. A result of the electron microscopic investigation was confirmed by immunoblotting analysis. Using MALDI Q-TOF mass spectrometry and Mascot program, the nuclear FBPase was identified as muscle chicken FBPase. FBPase activity in isolated cardiomyocyte nuclei was 5.9 mU/g. Nuclear FBPase was strongly inhibited by allosteric inhibitor AMP. I0.5 for AMP was 0.16 μM and was the same as for the purified chicken muscle enzyme.
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Authors
Monika Zmojdzian, Daria Dziewulska-Szwajkowska, Andrzej Dzugaj,