Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10821643 | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | 2012 | 7 Pages |
Abstract
The aim of this study was to evaluate the anesthesia induction and recovery times of sub-adult and post-larvae white shrimp (Litopenaeus vannamei) that were treated with eugenol and the essential oils (EOs) from Lippia alba and Aloysia triphylla. Oxidative stress parameters in the hemolymph of this species were also analyzed. The concentrations of eugenol, A. triphylla EO and L. alba EO recommended for anesthesia were 200, 300 and 750 μL Lâ 1 for sub-adults and 175, 300 and 500 μL Lâ 1 for post-larvae, respectively. The concentrations studied during the transport of sub-adults were between 20 and 50 μL Lâ 1 eugenol, 20-30 μL Lâ 1A. triphylla EO and 50 μL Lâ 1L. alba EO. For post-larvae, the optimal concentrations for transport were 20 μL Lâ 1 eugenol and between 20 and 50 μL Lâ 1A. triphylla EO. The white shrimp sub-adults that were exposed to A. triphylla EO (20 μL Lâ 1) showed increases in their total antioxidant capacities (150%), catalase (70%) and glutathione-S-transferase (615%) activity after 6 h. L. alba EO (50 μL Lâ 1) and eugenol (20 μL Lâ 1) also increased GST activity (1292 and 1315%) after 6 h, and eugenol (20 μL Lâ 1) decreased the total antioxidant capacity (100%). Moreover, concentrations above 30 μL Lâ 1 for the EOs of A. triphylla and L. alba and 20 μL Lâ 1 eugenol were effective at inducing anesthesia and improving the antioxidant system against reactive oxygen species (ROS) after 6 h.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Thaylise V. Parodi, Mauro A. Cunha, Clarissa G. Heldwein, Diego M. de Souza, Átila Clivea Martins, Luciano de O. Garcia, Wilson Wasielesky Junior, José MarÃa Monserrat, Denise Schmidt, Braulio O. Caron, Berta Heinzmann, Bernardo Baldisserotto,