Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10821661 | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | 2012 | 10 Pages |
Abstract
Mitochondrial respiration, activities of electron transport chain enzymes and formation of oxidative stress parameters were investigated in mitochondria isolated from gill tissue of mud crabs (Scylla serrata) as a function of salinity (10Â ppt, 17Â ppt and 35Â ppt). Mitochondrial oxygen consumption rate was higher for succinate as substrate compared with those of glutamate, malate and pyruvate. Complex I and complex II mediated respirations were higher at low salinity (10Â ppt) than high salinity (17Â ppt and 35Â ppt). Although activities of electron transport chain enzymes particularly complexes I (EC 1.6.5.3), II (EC 1.3.99.1) and II-III (EC 1.3.2.1) were elevated linearly in response to salinity treatment, activity of complex V (ATPase, EC 3.6.1.34) was decreased at 35Â ppt salinity. However, ATPase activity was higher at 17Â ppt salinity in comparison to 10Â ppt and 17Â ppt salinity. Results of the experiment suggest that high salinity (35Â ppt) causes hypoxic state in mitochondria of mud crabs. Hypoxic condition induced by high salinity was accompanied with increased hydrogen peroxide production resulting oxidative stress in mitochondria of crabs. A possible mechanism of hypoxia-induced reactive oxygen species generation and OS due to salinity stress in the crabs is discussed.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Biswaranjan Paital, G.B.N. Chainy,