Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10822547 | Current Opinion in Structural Biology | 2013 | 12 Pages |
Abstract
⺠Single-molecule spectroscopy can now be used to probe timescales from nanoseconds to weeks. ⺠Nanosecond-FCS allows the investigation of the submicrosecond dynamics of unfolded and intrinsically disordered proteins. ⺠Microfluidic mixing methods provide nonequilibrium single-molecule information from submillisecond to second timescales. ⺠The rigorous analysis of photon statistics has allowed the average transition path time of protein folding to be measured. ⺠Single-molecule spectroscopy starts to bridge the gap between folding in vitro and in vivo.
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Authors
Benjamin Schuler, Hagen Hofmann,