Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10822734 | Current Opinion in Structural Biology | 2005 | 6 Pages |
Abstract
Directed evolution is a common technique to engineer enzymes for a diverse set of applications. Structural information and an understanding of how proteins respond to mutation and recombination are being used to develop improved directed evolution strategies by increasing the probability that mutant sequences have the desired properties. Strategies that target mutagenesis to particular regions of a protein or use recombination to introduce large sequence changes can complement full-gene random mutagenesis and pave the way to achieving ever more ambitious enzyme engineering goals.
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Authors
Jesse D Bloom, Michelle M Meyer, Peter Meinhold, Christopher R Otey, Derek MacMillan, Frances H Arnold,