Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10822876 | Current Opinion in Structural Biology | 2005 | 11 Pages |
Abstract
The identification and characterization of protein interactions is a key topic in current life science research; a huge variety of methodologies have been established in recent years to expedite research in this area. Generic methods have been established for monitoring protein interactions in vivo by protein fragment complementation and for screening protein interactions in vitro by highly parallel solid-phase techniques. Substantial progress has been made in identifying and characterizing interactions with and between membrane proteins. Studying protein interactions on the single-molecule level has become an important tool for understanding protein function in vivo and in vitro.
Keywords
AFMDBDGFPY2HGPCRYFPTIRFG-protein-coupled receptorPCAProtein fragment complementation assayFluorescence resonance energy transferbioluminescence resonance energy transferFRETBRETTADSPRSurface plasmon resonanceTandem affinity purificationDNA-Binding Domaintranscription activation domainTAPyeast two-hybridyellow fluorescent proteingreen fluorescent proteinRiftotal internal reflection fluorescence
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Authors
Jacob Piehler,