Recruitment and retention dynamics of RECQL5 at DNA double strand break sites

Article ID	Journal	Published Year	Pages	File Type
10823420	DNA Repair	2012	12 Pages	PDF

Abstract

âº DNA double strand breaks (DSBs) were specifically generated using micro-irradiation. âº RECQL5 depleted cells accumulate persistent DSBs following Î³-irradiation. âº We provide insights into the recruitment and retention dynamics of RECQL5 at DSBs. âº Both the helicase and KIX domains of RECQL5 confer its recruitment and stable retention at DSBs. âº Human RecQ helicases have different inherent capacities to be retained at DSB sites.

Keywords

RTS BLM DSBs WRN RECQL5 Rothmund–Thomson syndrome Micro-irradiation SSBs Bloom syndrome Werner syndrome single strand breaks double strand breaks Confocal laser microscopy Homologous recombination Werner syndrome protein

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

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Recruitment and retention dynamics of RECQL5 at DNA double strand break sites

Authors

Venkateswarlu Popuri, Mahesh Ramamoorthy, Takashi Tadokoro, Dharmendra Kumar Singh, Parimal Karmakar, Deborah L. Croteau, Vilhelm A. Bohr,