| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10823464 | DNA Repair | 2012 | 10 Pages |
Abstract
⺠We examine biochemical and cellular properties of tumor-specific Pol β variant D160N. ⺠D160N is an active polymerase that binds DNA and functions in base excision repair. ⺠D160N expression in vivo results in cellular transformation. ⺠D160N is a mutator that generates template-directed complex tandem mutations.
Keywords
PBSpolymerase betaNei-likeNEILHerpes simplex virus-thymidine kinaseAP endonuclease 1WCEUDGFUdRdeoxyribose phosphateXRCC1HSV-tkAPE1HYGKSSdNTPBERSDSPAGEFPLCMEFDTTTETDRP5-fluoro-2′-deoxyuridineapurinic/apyrimidinicDNA polymerase βUracil DNA glycosylasepolyacrylamide gel electrophoresisTetracyclineequilibrium dissociation constantbase excision repairobserved rate constantdeoxynucleotide triphosphatedithiothreitolcircular dichroismsodium dodecyl sulfateCancerwhole cell extractfast performance liquid chromatographyPhosphate buffered salinemouse embryonic fibroblastwild-typehygromycinpol βChloramphenicolkobs
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Katherine A. Donigan, Suzanne E. Hile, Kristin A. Eckert, Joann B. Sweasy,