Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10824937 | Journal of Biochemical and Biophysical Methods | 2005 | 5 Pages |
Abstract
A PCR method for uniform amplification of a random sequence DNA library is described. A combination of 1 M betaine and 5% DMSO improves the PCR amplification by increasing the ratio of full-length products to shortened products, which are a consequence of nonuniform amplification due to stable secondary structures in the templates. This method is expected to be beneficial for obtaining high-affinity aptamers with stable secondary structures.
Keywords
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Authors
Jonghoon Kang, Myung Soog Lee, David G. Gorenstein,