Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10824942 | Journal of Biochemical and Biophysical Methods | 2005 | 12 Pages |
Abstract
The interaction of DNA and human serum albumin (HSA) in the presence of anticancer drug carboplatin was studied with piezoelectric quartz crystal impedance (PQCI) and electrochemistry techniques. In the PQCI analysis, the correlative parameters including the frequency (f0), the motional resistance (Rm), and the static capacitance (C0) in the experiment were obtained and discussed in detail. Additionally, the kinetics parameters of the cross-linking process were investigated and a response kinetics model was deduced. The values of association rate constant k1, dissociation rate constant kâ1 and the reaction equilibrium constant K were estimated to be 1.895 (mg/ml)â1 sâ1, 4.7Â ÃÂ 10â5 sâ1 and 4.03Â ÃÂ 104 (mg/ml)â1, respectively. Furthermore, cyclic voltammetry (CV) and electrochemical AC impedance techniques were employed to testify the cross-linking process.
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Authors
Shanchao Liu, Yingju Liu, Jia Li, Manli Guo, Lihua Nie, Shouzhuo Yao,