Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825115 | Journal of Biochemical and Biophysical Methods | 2005 | 6 Pages |
Abstract
Neuronal growth inhibitory factor (GIF) is a small cysteine-rich metal binding protein downregulated in Alzheimer's disease. The protein belongs to the superfamily of metallothioneins (MTs) and was classified as MT-3. Although first identified as a brain specific protein, several reports now indicate a substantially broader expression pattern. However, currently available detection methods for MT-3 show low sensitivity in gel electrophoresis and Western blot. We have developed a fast and sensitive method for MT-3 detection in SDS-PAGE (detection limit â¼10 ng) and Western blot (detection limit â¼1 ng). The method is based on the chemical modification of cysteine residues with the dye monobromobimane and an improved blotting protocol.
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Authors
Gabriele Meloni, Markus Knipp, Milan VaÅ¡ák,