Article ID Journal Published Year Pages File Type
10825115 Journal of Biochemical and Biophysical Methods 2005 6 Pages PDF
Abstract
Neuronal growth inhibitory factor (GIF) is a small cysteine-rich metal binding protein downregulated in Alzheimer's disease. The protein belongs to the superfamily of metallothioneins (MTs) and was classified as MT-3. Although first identified as a brain specific protein, several reports now indicate a substantially broader expression pattern. However, currently available detection methods for MT-3 show low sensitivity in gel electrophoresis and Western blot. We have developed a fast and sensitive method for MT-3 detection in SDS-PAGE (detection limit ∼10 ng) and Western blot (detection limit ∼1 ng). The method is based on the chemical modification of cysteine residues with the dye monobromobimane and an improved blotting protocol.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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