Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825733 | Methods | 2014 | 7 Pages |
Abstract
- A multiphoton cryomicroscope based on a low-power femtosecond fiber laser was built.
- Multiphoton sectioning can be performed within ice.
- Autofluorescence intensity and fluorescence lifetime increase during freezing.
- Label-free optical biopsies of cryopreserved biomaterial can be obtained.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biochemistry
Authors
Karsten König, Aisada Uchugonova, Hans Georg Breunig,