Article ID Journal Published Year Pages File Type
10825733 Methods 2014 7 Pages PDF
Abstract

- A multiphoton cryomicroscope based on a low-power femtosecond fiber laser was built.
- Multiphoton sectioning can be performed within ice.
- Autofluorescence intensity and fluorescence lifetime increase during freezing.
- Label-free optical biopsies of cryopreserved biomaterial can be obtained.
Related Topics
Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry
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