Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825847 | Methods | 2013 | 5 Pages |
Abstract
We report herein a luminescent switch-on label-free G-quadruplex-based assay for the rapid and sensitive detection of polymerase proofreading activity using a novel iridium(III) complex as a G-quadruplex-selective probe. The interaction of the iridium(III) complex with the G-quadruplex motif facilitates the highly sensitive switch-on detection of polymerase proofreading activity. Using T4 DNA polymerase (T4 pol) as a model enzyme, the assay achieved high sensitivity and selectivity for T4 pol over other tested enzymes.
Keywords
deoxyadenosine monophosphateT4 DNA polymeraseExonuclease IExonuclease IIIT7 ExonucleaseExo IIIdGMPssDNADeoxyguanosine monophosphatedNTPG-quadruplexDAMPCtDNAACNdsDNASingle-stranded DNAcalf-thymus DNAdouble-stranded DNAAcetonitrileTaq DNA polymerasedeoxyribonucleoside triphosphateLuminescentProofreadingpolymerase chain reactionPCRPolymeraseIridium(III) complex
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Authors
Ka-Ho Leung, Hong-Zhang He, Hai-Jing Zhong, Lihua Lu, Daniel Shiu-Hin Chan, Dik-Lung Ma, Chung-Hang Leung,