Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10825872 | Methods | 2013 | 9 Pages |
Abstract
The increasing use of zebrafish larvae for biomedical research applications is resulting in versatile models for a variety of human diseases. These models exploit the optical transparency of zebrafish larvae and the availability of a large genetic tool box. Here we present detailed protocols for the robotic injection of zebrafish embryos at very high accuracy with a speed of up to 2000 embryos per hour. These protocols are benchmarked for several applications: (1) the injection of DNA for obtaining transgenic animals, (2) the injection of antisense morpholinos that can be used for gene knock-down, (3) the injection of microbes for studying infectious disease, and (4) the injection of human cancer cells as a model for tumor progression. We show examples of how the injected embryos can be screened at high-throughput level using fluorescence analysis. Our methods open up new avenues for the use of zebrafish larvae for large compound screens in the search for new medicines.
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Authors
Herman P. Spaink, Chao Cui, Malgorzata I. Wiweger, Hans J. Jansen, Wouter J. Veneman, Rubén MarÃn-Juez, Jan de Sonneville, Anita Ordas, Vincenzo Torraca, Wietske van der Ent, William P. Leenders, Annemarie H. Meijer, B. Ewa Snaar-Jagalska,