Solid-phase capture for the detection and relative quantification of S-nitrosoproteins by mass spectrometry

Article ID	Journal	Published Year	Pages	File Type
10825881	Methods	2013	8 Pages	PDF

Abstract

âº We describe critical parameters for analysis of S-nitrosylated proteins by SNO-RAC. âº We give methods for MS-based identification of S-nitrosylated proteins by SNO-RAC. âº We review the tractable MS-based methods for relative quantitation by SNO-RAC.

Keywords

S-nitrosoglutathione CysNO S-nitrosocysteine S-nitrosylation ITRAQ S-nitrosothiol SNO GSNO TMT Palmitoylation LC-MS/MS Tandem mass tag isobaric tags for relative and absolute quantitation Redox SILAC Mass spectrometry Nitric oxide Proteomics liquid chromatography tandem mass spectrometry

Related Topics

Life Sciences Biochemistry, Genetics and Molecular Biology Biochemistry

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Solid-phase capture for the detection and relative quantification of S-nitrosoproteins by mass spectrometry

Authors

J. Will Thompson, Michael T. Forrester, M. Arthur Moseley, Matthew W. Foster,