Functional proteomics approaches for the identification of transnitrosylase and denitrosylase targets

Article ID	Journal	Published Year	Pages	File Type
10825883	Methods	2013	35 Pages	PDF

Abstract

âº Quantitative proteomics methods can measure changes of peptide S-nitrosylation status. âº Transnitrosylases catalyze increased S-nitrosylation of specific peptides. âº Denitrosylases catalyze decreased S-nitrosylation of specific peptides. âº Quantitative proteomics information can be used to characterize transnitrosylase and denitrosylase activities.

Keywords

Trx reductase MRM ICAT IEF TMT GSNO S-nitrosoglutathione Trx TrxR BST ITRAQ Biotin-HPDP DDA S-nitrosylation MMTS Biotin-switch technique binding buffer ESI TFA BCA CID FDR PTM GAPDH NADPH ACN 2DE MS/MS Collision-induced dissociation Acetonitrile Trifluoroacetic acid post-translational modification two-dimensional gel electrophoresis Lysis buffer isobaric tag for relative and absolute quantitation stable isotope labeling with amino acids in cell culture bicinchoninic acid isoelectric focusing Tandem mass tags Isotope-coded affinity tags thioredoxin Biotin switch SILAC Tandem mass spectrometry MALDI methyl methanethiosulfonate false discovery rate Multiple-reaction monitoring nicotinamide adenine dinucleotide phosphate Proteomics glyceraldehyde 3-phosphate dehydrogenase electrospray ionization matrix-assisted laser desorption ionization